火炭母药材的DNA条形码分子鉴定研究

侯典云,杨萌萌,李炯,马占强,周爽,赵杏利,胥华伟,张改娜

中国药学杂志 ›› 2018, Vol. 53 ›› Issue (14) : 1165-1169.

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中国药学杂志
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中国药学杂志 ›› 2018, Vol. 53 ›› Issue (14) : 1165-1169. DOI: 10.11669/cpj.2018.14.005
论著

火炭母药材的DNA条形码分子鉴定研究

  • 侯典云1,2,杨萌萌1,2,李炯3,马占强1,周爽1,2,赵杏利1,2,胥华伟1,2,张改娜1
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Molecular Identification of Polygonum chinensis Using DNA Barcoding

  • HOU Dian-yun1,2, YANG Meng-meng1,2, LI Jiong3,MA Zhan-qiang1, ZHOU Shuang1,2, ZHAO Xing-li1,2,XU Hua-wei1,2, ZHANG Gai-na1
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摘要

目的 通过ITS2序列鉴别火炭母药材及其混伪品。方法 试剂盒法提取火炭母药材基因组DNA,PCR扩增其ITS2序列,MEGA6.0软件分析供试材料序列的变异位点,计算其种内种间K2P遗传距离,构建NJ系统聚类树。结果 火炭母药材种内变异小,与其混伪品ITS2序列的二级结构差异显著,系统聚类树可明显区分火炭母药材及其混伪品。结论 ITS2条形码可用于火炭母药材及其常见混伪品的鉴别,丰富了火炭母药材鉴定的技术方法,确定了火炭母药材的标准DNA条形码序列,对火炭母药材的准确鉴定和临床用药安全奠定了基础。

Abstract

OBJECTIVE To identify Polygonum chinensis and its adulterants by ITS2 sequences. METHODS Total genomic DNA of P. chinensis was extracted using the plant genomic DNA kit. The internal transcribed spacer 2(ITS2) regions were amplified. The variable site of ITS2 regions were analysed through MEGA 6.0 software. The intra-versus inter-specific genetic distances of the ITS2 regions was calculated based on the kimura 2-parameter(K2P) model. Neighbor-Joining phylogenetic trees were constructed using MEGA6.0. RESULTS The intraspecific variation of P. chinensis was small. However, the interspecific variation of P. chinensis and its adulterants was small distinct. The secondary structure of ITS2 of P. chinensis and its adulterants has significant difference. NJ trees can identify P. chinensis and its adulterants. CONCLUSION ITS2 Regions can be used to authenticate P. chinensis and its adulterants which provide new METHODS for the identification of P. chinensis. The standard DNA barcodes of P. chinensis are establishment which would lay the foundation of identification and safety clinical drug application of P. chinensis.

关键词

火炭母 / ITS2序列 / DNA条形码 / 分子鉴定

Key words

Polygonum chinensis / ITS2 region / DNA barcode / molecular identification

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侯典云,杨萌萌,李炯,马占强,周爽,赵杏利,胥华伟,张改娜. 火炭母药材的DNA条形码分子鉴定研究[J]. 中国药学杂志, 2018, 53(14): 1165-1169 https://doi.org/10.11669/cpj.2018.14.005
HOU Dian-yun, YANG Meng-meng, LI Jiong,MA Zhan-qiang, ZHOU Shuang, ZHAO Xing-li,XU Hua-wei, ZHANG Gai-na. Molecular Identification of Polygonum chinensis Using DNA Barcoding[J]. Chinese Pharmaceutical Journal, 2018, 53(14): 1165-1169 https://doi.org/10.11669/cpj.2018.14.005
中图分类号： R285   

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基金

国家自然科学基金项目资助(U1404829);河南省教育厅自然科学研究计划重点项目资助(12B180007,14A180006)
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